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Urinary 8-epi-Prostaglandin F2a Assay Kit
Test Urinary 8-epiâ-âProstaglandin F2a je kompetitivnĂ enzymovĂ˝ imunoanalĂ˝za (ELISA) pro stanovenĂ hladin 8-epiâ-âprostaglandinuâ-âF2a v moÄovĂ˝ch vzorcĂch. StruÄnÄ ĹeÄeno, vzorky se smĂchajĂ s zesilujĂcĂm Äinidlem, kterĂŠ v podstatÄ eliminuje interference zpĹŻsobenĂŠ nespecifickou vazbou. 8-epiâ-âprostaglandinâ-âF2a ve vzorku nebo standardu pak soutÄŞà s 8-epiâ-âprostaglandinemâ-âF2a konjugovanĂ˝m s kĹenovou peroxidĂĄzou (HRP konjugĂĄt) o vazbu na polyklonĂĄlnĂ protilĂĄtku specifickou pro 8-epiâ-âprostaglandinâ-âF2a potaĹženou na mikrotitraÄnĂ destiÄce. Po pĹidĂĄnĂ substrĂĄtu je intenzita barvy nepĹĂmo ĂşmÄrnĂĄ mnoĹžstvĂ nekonjugovanĂŠho 8-epiâ-âprostaglandinuâ-âF2a ve vzorku nebo standardu.
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